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cy3 streptavidin incubation  (Jackson Immuno)


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    Structured Review

    Jackson Immuno cy3 streptavidin incubation
    Cy3 Streptavidin Incubation, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1771 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy3+streptavidin+incubation/Cy+3+Streptavidin/pmc13038874-73-39-43
    Average 96 stars, based on 1771 article reviews
    cy3 streptavidin incubation - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Teneurin-2 and related proteins in reactive astrocytes after status epilepticus induction in adult rats
    Article Snippet: Next, the sections were incubated in a solution of primary polyclonal antibody anti-Ten-2 (1:100, Lot K1910, sc-165674, Santa Cruz Biotechnology, CA, USA) or ADGRL1 (1:1000, Lot ALR021AN0102, ALR-021, Alomone Labs, Jerusalem, Israel) PBS-TX and 2% normal donkey serum for 48 h at 4 °C with slow agitation. .. After this step, the sections were incubated in the specific biotinylated secondary antibody (1:800, lot #G0815, sc-2042, Santa Cruz Biotechnology or 1:500, lot #1515108, 711–065-152, Jackson Immunoresearch Laboratories Inc., PA, USA) for 90 min at 4° C followed by Cy3-streptavidin incubation (1:500, #016–160-084, Jackson Immunoresearch, PA, USA) for 60 min at 4 °C. .. Next, the sections were washed in PBS and dipped in a solution with PBS-TX, 2% normal donkey serum and primary polyclonal antibody anti-GFAP (1:250, Lot #2145934, AB5804, EMD Millipore, MA, USA or 1;500, Lot #2987471, AB5541, EMD Millipore Corporation, CA, USA), overnight at 4 °C.



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